9UIB

Crystal Structure of Oryza sativa SGT1-TPR domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 3
Unit cell lengths	93.493, 93.493, 32.941
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.370 - 1.530
R-factor	0.1766
Rwork	0.176
R-free	0.19040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7obe
RMSD bond length	0.006
RMSD bond angle	0.838
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.370	1.560
High resolution limit [Å]	1.530	1.530
Number of reflections	48742	4851
<I/σ(I)>	48.83
Completeness [%]	100.0
Redundancy	18.9
CC(1/2)	0.998	0.879

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		300	Magnesium chloride hexahydrate, Tris, PEG3350