9UI2
Crystal structure of Thermus thermophilus HB8 transaldolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-03-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 173.380, 173.380, 422.580 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.750 - 1.700 |
| R-factor | 0.19051 |
| Rwork | 0.189 |
| R-free | 0.21109 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3s1v |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.693 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.750 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.120 | |
| Number of reflections | 402504 | 29603 |
| <I/σ(I)> | 17.4 | 2.4 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 20 | |
| CC(1/2) | 0.997 | 0.890 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.26 M (NH4)SO4, 0.2 M LiSO4, 0.1 M Tris-HCl pH8.5 |
