9UGH
crystal structure of ferritin from pig spleen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-11-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 181.020, 181.020, 181.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.290 - 2.310 |
| R-factor | 0.2071 |
| Rwork | 0.202 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.102 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.605 | 2.393 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Number of reflections | 11667 | 1122 |
| <I/σ(I)> | 8.51 | |
| Completeness [%] | 99.8 | |
| Redundancy | 2 | |
| CC(1/2) | 0.995 | 0.641 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.05 M Cadmium sulfate hydrate 0.1 M HEPES pH 7.5 1.0 M Sodium acetate trihydrate |
