9UGE

Crystal structure of the complex of camel peptidoglycan recognition protein, PGRP-S with malic acid and oxalic acid at 2.3 A resolution

Replaces:  4Q9E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	77
Detector technology	CCD
Collection date	2014-02-12
Detector	MARRESEARCH
Wavelength(s)	0.97
Spacegroup name	I 2 2 2
Unit cell lengths	88.308, 101.623, 162.683
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.704 - 2.305
Rwork	0.215
R-free	0.28330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.235
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.704	2.340
High resolution limit [Å]	2.305	2.305
Number of reflections	29076	797
<I/σ(I)>	9.8
Completeness [%]	89.2
Redundancy	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	298	20% glycerol, 6% PEG3350