9UFQ
Crystal structure of L-asparaginase from Thermococcus Sibiricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-26 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 45.193, 57.791, 131.158 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.110 - 1.900 |
| R-factor | 0.16944 |
| Rwork | 0.167 |
| R-free | 0.21445 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ot0 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.799 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.110 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.080 | 0.308 |
| Rmeas | 0.084 | 0.321 |
| Rpim | 0.025 | 0.088 |
| Total number of observations | 295225 | 22284 |
| Number of reflections | 26391 | 1693 |
| <I/σ(I)> | 21.7 | 7.8 |
| Completeness [%] | 95.3 | |
| Redundancy | 11.2 | 13.2 |
| CC(1/2) | 0.999 | 0.975 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.2 M Ammonium acetate, 20% w/v Polyethylene glycol 3,350 |
