9UFI

Crystal structure of a PhGs rhamnosyltransferase UGT79G15 from Rehmannia glutinosa in complex with UDP and FSA

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-01-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	46.105, 73.244, 134.294
Unit cell angles	90.00, 97.52, 90.00

Refinement procedure

Resolution	49.260 - 2.120
R-factor	0.2021
Rwork	0.200
R-free	0.24760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.115
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.260	2.190
High resolution limit [Å]	2.120	2.120
Number of reflections	49883	4995
<I/σ(I)>	56
Completeness [%]	98.6
Redundancy	14.87
CC(1/2)	0.996	0.652

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	0. 4 M NaNO3,30% PEG 3350