9UFG

Structure of a triple-helix region of human collagen type XII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Collection date	2025-04-07
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54187
Spacegroup name	P 21 21 21
Unit cell lengths	14.119, 45.229, 88.123
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.240 - 1.900
R-factor	0.1841
Rwork	0.181
R-free	0.24630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.922
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.240	40.240
High resolution limit [Å]	1.900	1.900
Rmerge	0.104
Number of reflections	4948	295
<I/σ(I)>	21.1
Completeness [%]	99.9
Redundancy	12.3
CC(1/2)	0.998	0.909

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2M NH4NO3, Ammonium Nitrate, PH 6.3