9UF7

Crystal structure of a PhGs rhamnosyltransferase UGT79G15 from Rehmannia glutinosa in complex with UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9785
Spacegroup name	P 1 21 1
Unit cell lengths	46.254, 72.059, 134.360
Unit cell angles	90.00, 97.83, 90.00

Refinement procedure

Resolution	48.890 - 2.400
R-factor	0.208
Rwork	0.206
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.279
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.890	2.480
High resolution limit [Å]	2.400	2.400
Number of reflections	34485	3402
<I/σ(I)>	1.91
Completeness [%]	99.9
Redundancy	4.09
CC(1/2)	0.981	0.591

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	0. 4 M NaNO3,30% PEG 3350