9UDN

Single-chain Fv antibody of F8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	135.730, 35.140, 46.450
Unit cell angles	90.00, 99.63, 90.00

Refinement procedure

Resolution	45.800 - 1.810
R-factor	0.1825
Rwork	0.180
R-free	0.22030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.436
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.920
High resolution limit [Å]	1.810	1.810
Rmerge	0.166	1.545
Number of reflections	19851	3194
<I/σ(I)>	7.8	1.4
Completeness [%]	99.5
Redundancy	6.6
CC(1/2)		0.595

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M HEPES sodium pH 7.5, 1.4 M sodium citrate tribasic dihydrate