9UDI
Cryo-EM structure of the RdCas12n-sgRNA-DNA ternary complex, Conformation 2
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | TFS FALCON 4i (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.01 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
