9UDH

bacteriophage lytic transglycosidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	113
Detector technology	PIXEL
Collection date	2024-05-31
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.987
Spacegroup name	C 2 2 21
Unit cell lengths	67.727, 89.583, 67.739
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.290 - 1.850
R-factor	0.1965
Rwork	0.194
R-free	0.21540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold
RMSD bond length	0.007
RMSD bond angle	0.901
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.880
High resolution limit [Å]	1.850	1.850
Number of reflections	18434	710
<I/σ(I)>	15.9
Completeness [%]	98.7
Redundancy	6.3
CC(1/2)	0.991	0.867

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	peg 3350, magnesium formate