9UCN
Monomer of SARS-CoV-2 nsp4CTD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-09 |
| Detector | STFC Large Pixel Detector |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 56.880, 56.880, 78.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.440 - 1.440 |
| R-factor | 0.2104 |
| Rwork | 0.208 |
| R-free | 0.23640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.771 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX (1.20.1_4487) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.580 | 1.480 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Rmerge | 0.049 | 1.129 |
| Number of reflections | 26910 | 1888 |
| <I/σ(I)> | 1.55 | |
| Completeness [%] | 99.9 | |
| Redundancy | 1.55 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.8 M lithium sulfate monohydrate, 0.1 M sodium acetate trihydrate, at pH 4.6. |
