9UBD

The Structural Basis of the Recognition of the Histone Variant H2A.Z by the SRCAP Catalytic Subunit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	77.330, 102.190, 67.370
Unit cell angles	90.00, 114.78, 90.00

Refinement procedure

Resolution	35.100 - 2.530
R-factor	0.2537
Rwork	0.252
R-free	0.28840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.022
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX (1.20.1_4487_000)
Refinement software	PHENIX ((1.20.1_4487_000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.100	2.600
High resolution limit [Å]	2.530	2.530
Rmerge	0.129	0.645
Rmeas	0.142	0.705
Rpim	0.057	0.280
Number of reflections	14736	1171
<I/σ(I)>	10.8	2.6
Completeness [%]	92.7
Redundancy	6.4
CC(1/2)	0.959	0.875

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.2 M Potassium thiocyanate 15 % w/v PEG 3,350 pH 7.0