9UAY
Crystal structure of CmnI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2025-03-12 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 167.888, 129.520, 61.313 |
| Unit cell angles | 90.00, 95.15, 90.00 |
Refinement procedure
| Resolution | 27.650 - 2.170 |
| R-factor | 0.21644 |
| Rwork | 0.215 |
| R-free | 0.24552 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.292 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.650 | 2.250 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Number of reflections | 61605 | 6681 |
| <I/σ(I)> | 20.8 | |
| Completeness [%] | 98.2 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% (w/v) PEG 1000 100 mM Tris base/ Hydrochloric acid pH 8.5 |
