9U7Z

5hmC specific restriction endonuclease Escherichia coli APEC O1 PD-T4-3 in complex with 5-Hydroxymethyl-dCTP

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 64 2 2
Unit cell lengths	124.728, 124.728, 80.796
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.368 - 2.153
R-factor	0.2325
Rwork	0.230
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.846
Data reduction software	HKL-3000 (7.21)
Data scaling software	HKL-3000 (7.21)
Phasing software	PHASER (2.7.0)
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.368	2.190
High resolution limit [Å]	2.153	2.153
Number of reflections	20652	979
<I/σ(I)>	37.75
Completeness [%]	99.1
Redundancy	38.1
CC(1/2)	0.975	0.723

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.9	293.15	0.49 M Sodium phosphate monobasic monohydrate, 0.91 M Potassium phosphate dibasic, pH 6.9