9U6H
Cryo-EM structure of the Vo domain of V/A-ATPase in liposomes, state2
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | JEOL CRYO ARM 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | In-column Omega Filter |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3 |
| Number of Particle | 2094178 |
| Software | Topaz,PHENIX,cryoSPARC,CTFFIND,Coot,ISOLDE |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
