9U49

crystal structure of Cdn1 in complex with cA4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 2
Unit cell lengths	89.930, 132.440, 75.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.930 - 1.900
R-factor	0.19
Rwork	0.189
R-free	0.22670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.855
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.150	1.830
High resolution limit [Å]	1.780	1.780
Rmerge	0.120	1.567
Rmeas	0.125	1.629
Rpim	0.035	0.442
Total number of observations	1121701	85828
Number of reflections	87463	6398
<I/σ(I)>	15.8	2.8
Completeness [%]	99.9
Redundancy	12.8	13.4
CC(1/2)	0.997	0.824

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288.15	18% PEG 8000(w/v), 0.1M Tris-HCl pH7.5, 0.2M MgCl2