9U46

The X-ray structure of N-terminal catalytic domain of Thermoplasma acidophilum tRNA methyltransferase Trm56 (Ta0931) in complex with 5'-Methylthioadenosine

Replaces:  8X6J

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	46.700, 71.400, 86.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.083 - 1.992
Rwork	0.171
R-free	0.22430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yy8
RMSD bond length	0.008
RMSD bond angle	1.595
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.110
High resolution limit [Å]	1.990	1.990
Rmerge	0.069	0.280
Number of reflections	10241	1613
<I/σ(I)>	22.19	8.57
Completeness [%]	99.4	98
Redundancy	5.9
CC(1/2)	1.000	0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	PEG3350, Bis-Tris