9U3F
Monomeric sarcosine oxidase from Bacillus sp. (SoxB) Y254G mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.580, 69.673, 73.676 |
| Unit cell angles | 90.00, 93.73, 90.00 |
Refinement procedure
| Resolution | 42.364 - 1.450 |
| Rwork | 0.160 |
| R-free | 0.18570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1el5 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.872 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.364 | 1.470 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.078 | 0.777 |
| Number of reflections | 128302 | 6356 |
| <I/σ(I)> | 11.4 | 2.6 |
| Completeness [%] | 99.0 | |
| Redundancy | 5.4 | |
| CC(1/2) | 0.998 | 0.765 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.8 M Ammonium sulfate, 100 mM Tris-HCl buffer pH8.5 |
