9U3C
Monomeric sarcosine oxidase from Bacillus sp. (SoxB) complexed with L-Proline
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.674, 69.049, 73.966 |
| Unit cell angles | 90.00, 93.97, 90.00 |
Refinement procedure
| Resolution | 40.504 - 1.540 |
| Rwork | 0.149 |
| R-free | 0.17610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1el5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.788 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.340 | 1.570 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.090 | 0.984 |
| Number of reflections | 107719 | 5287 |
| <I/σ(I)> | 7.5 | 2.3 |
| Completeness [%] | 99.8 | |
| Redundancy | 3.9 | |
| CC(1/2) | 0.995 | 0.713 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.5 M Ammonium sulfate, 100 mM Tris-HCl buffer pH8.5 |
