9U2X
Crystal structure of N244L variant from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-01-29 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.873128 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 351.931, 73.875, 127.231 |
| Unit cell angles | 90.00, 110.74, 90.00 |
Refinement procedure
| Resolution | 83.410 - 2.670 |
| R-factor | 0.2998 |
| Rwork | 0.297 |
| R-free | 0.34800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.855 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.411 | 3.064 |
| High resolution limit [Å] | 2.669 | 2.669 |
| Rpim | 0.122 | 0.040 |
| Number of reflections | 41663 | 2083 |
| <I/σ(I)> | 4.6 | |
| Completeness [%] | 90.7 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Ammonium nitrate 0.1 M HEPES 7.5 20 % v/v PEG Smear Broad |
