9TZZ

Crystal structure of NbC03, a nanobody against the PWWP domains of LEDGF and HRP-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.87313
Spacegroup name	P 43 21 2
Unit cell lengths	72.672, 72.672, 109.618
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.570 - 2.930
R-factor	0.2654
Rwork	0.262
R-free	0.32150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.468
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	109.618	3.690
High resolution limit [Å]	2.927	2.930
Number of reflections	144639	2360
<I/σ(I)>	4
Completeness [%]	91.5
Redundancy	24.2
CC(1/2)	0.822	0.493

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.5 M Ammonium sulfate 12% Glycerol 0.1 M Tris-HCl pH 8.5