9TZ4

Crystal structure of the outer membrane lipoprotein OprI from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-07-10
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.8731
Spacegroup name	P 21 3
Unit cell lengths	52.600, 52.600, 52.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.194 - 2.101
Rwork	0.254
R-free	0.26670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.219
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.600	2.180
High resolution limit [Å]	2.100	2.100
Rpim	0.038
Number of reflections	2916	306
<I/σ(I)>	13
Completeness [%]	97.2	100
Redundancy	17.5
CC(1/2)		0.383

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	30% PEG 200, 0.1 M MES pH 6.5 and 0.1 M sodium acetate