9TXQ
Spinach Ferredoxin I, Reduced with Methyl Viologen at -450mV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-10-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.7085 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.220, 51.770, 61.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.690 - 1.100 |
| R-factor | 0.1678 |
| Rwork | 0.167 |
| R-free | 0.18510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.067 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.570 | 1.120 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.080 | 4.240 |
| Rpim | 0.023 | 1.223 |
| Number of reflections | 39926 | 1964 |
| <I/σ(I)> | 11.7 | 0.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.1 | 13 |
| CC(1/2) | 0.999 | 0.421 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 277 | 3.5M Ammonium sulfate, 0.1M Sodium phosphate pH 7.8, 5% Glycerol |
