9TXO
Spinach Ferredoxin I, Re-Oxidised
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-10-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.7085 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.232, 51.756, 61.309 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.540 - 1.200 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.975 |
| Data reduction software | xia2.multiplex |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.540 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.090 | 2.694 |
| Rpim | 0.025 | 0.742 |
| Number of reflections | 30858 | 1484 |
| <I/σ(I)> | 11.8 | 0.4 |
| Completeness [%] | 98.5 | |
| Redundancy | 14.1 | |
| CC(1/2) | 0.999 | 0.425 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 277 | 3.5M Ammonium Sulfate, 0.1M Sodium phosphate pH 7.8, 5% Glycerol |
