9TXE
Spinach Ferredoxin I, Oxidised
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.7749 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.265, 51.212, 61.169 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.580 - 0.950 |
| R-factor | 0.1646 |
| Rwork | 0.164 |
| R-free | 0.17840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.898 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.260 | 0.970 |
| High resolution limit [Å] | 0.950 | 0.950 |
| Rmerge | 0.056 | 1.490 |
| Rpim | 0.025 | 0.719 |
| Number of reflections | 60723 | 2948 |
| <I/σ(I)> | 11.3 | 0.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.1 | 5.2 |
| CC(1/2) | 0.999 | 0.489 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 3.5M Ammonium Sulfate, 0.1M Sodium phosphate pH 7.8, 5% Glycerol |
