9TWT
Complex of E. coli leucyl-tRNA synthetase AN3365-resistant mutant D251N bound to tRNA(leu) and AN3365
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-12 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9655 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.581, 72.323, 104.870 |
| Unit cell angles | 90.00, 115.30, 90.00 |
Refinement procedure
| Resolution | 83.220 - 2.299 |
| R-factor | 0.2032 |
| Rwork | 0.201 |
| R-free | 0.25620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.830 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.220 | 2.550 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rpim | 0.049 | 0.522 |
| Number of reflections | 40504 | 2026 |
| <I/σ(I)> | 11.2 | 1.5 |
| Completeness [%] | 93.4 | 60.5 |
| Redundancy | 5.2 | 5.6 |
| CC(1/2) | 0.997 | 0.545 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M KH2PO4, 19-23% PEG 3350 |
