9TWF

Putative Polyurethane Degrading Amidase Signature Family Amidase in Complex with PMSF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.7293
Spacegroup name	P 65
Unit cell lengths	134.780, 134.780, 64.150
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.180 - 1.890
R-factor	0.2096
Rwork	0.209
R-free	0.22760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.794
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	116.934	1.891
High resolution limit [Å]	1.859	1.859
Number of reflections	56358	2840
<I/σ(I)>	7.6
Completeness [%]	100.0
Redundancy	14.5
CC(1/2)	0.998	0.369

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Morpheus Screen A9