9TW8

Putative Polyurethane Degrading Amidase Signature Family Amidase in Ligand Free State

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-08-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.7293
Spacegroup name	P 65
Unit cell lengths	135.431, 135.431, 64.785
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.530 - 1.650
R-factor	0.1622
Rwork	0.162
R-free	0.17840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.045
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.720	1.673
High resolution limit [Å]	1.645	1.645
Number of reflections	82226	4115
<I/σ(I)>	10.4
Completeness [%]	100.0
Redundancy	15
CC(1/2)	0.990	0.360

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Morpheus Screen A9