9TUX

PfRIPR bound to monoclonal antibody MAD8-66

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-20
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	52.790, 63.240, 250.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.680 - 2.250
R-factor	0.232
Rwork	0.230
R-free	0.26220
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.250	2.290
High resolution limit [Å]	2.250	2.250
Number of reflections	549427	28167
<I/σ(I)>	8.3
Completeness [%]	100.0
Redundancy	13.4
CC(1/2)	0.996	0.415

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M sodium cacodylate pH 6.5, 40% w/v MPD, 5% w/v PEG 8000