9TUW

PfRIPR EGF6 and 7 bound to monoclonal antibody RP.012

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-24
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9537
Spacegroup name	P 1
Unit cell lengths	50.160, 50.983, 109.782
Unit cell angles	76.82, 83.26, 76.50

Refinement procedure

Resolution	48.660 - 2.196
R-factor	0.2421
Rwork	0.241
R-free	0.25570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.970
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	BUSTER (2.10.4 (10-JUL-2024))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.670	2.230
High resolution limit [Å]	2.196	2.196
Number of reflections	51504	2453
<I/σ(I)>	11.1
Completeness [%]	98.3
Redundancy	3.6
CC(1/2)	0.995	0.317

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Buffer Sytem 3 pH 8.5, 0.1 M Carboxylic acids, 37.5% v/v Precipitant Mix 4