9TU4
Uracil-DNA glycosylase from Micrococcus flavus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-30 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9679 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 59.276, 43.722, 84.511 |
| Unit cell angles | 90.00, 95.32, 90.00 |
Refinement procedure
| Resolution | 42.073 - 1.710 |
| R-factor | 0.16641 |
| Rwork | 0.165 |
| R-free | 0.20255 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.593 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0431) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.073 | 1.584 |
| High resolution limit [Å] | 1.482 | 1.482 |
| Rmerge | 0.119 | 1.095 |
| Rmeas | 0.130 | 1.210 |
| Rpim | 0.051 | 0.505 |
| Total number of observations | 178813 | 7855 |
| Number of reflections | 28705 | 1435 |
| <I/σ(I)> | 8.2 | 1.3 |
| Completeness [%] | 79.8 | |
| Redundancy | 6.2 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M Sodium HEPES pH 7.5, 10% v/v 2-Propanol, 20% w/v PEG 4000 |
