9TPP
Antibody-antigen complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.131, 61.685, 117.047 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.310 - 1.800 |
| R-factor | 0.1698 |
| Rwork | 0.168 |
| R-free | 0.20170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.849 |
| Data reduction software | XDS (20141103) |
| Data scaling software | Aimless (0.8.2) |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.700 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rpim | 0.047 | 0.261 |
| Number of reflections | 39614 | 2163 |
| <I/σ(I)> | 13.2 | 3.3 |
| Completeness [%] | 99.1 | |
| Redundancy | 4.7 | |
| CC(1/2) | 0.996 | 0.830 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 0.1M HEPES, pH 7.5, 18% PEG 3350, 20% isopropanol |
