9TPF
The structure of the orthorhombic crystal form of the type II ribosome inactivating protein from Winter Aconite Eranthis hyemalis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9282 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.589, 95.470, 96.339 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.735 - 1.800 |
| Rwork | 0.182 |
| R-free | 0.23330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | Servalcat (0.4.105) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.800 | 1.810 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.106 | 2.552 |
| Rmeas | 0.110 | 2.661 |
| Number of reflections | 72825 | 4058 |
| <I/σ(I)> | 14.8 | 1 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 13.3 | 12.5 |
| CC(1/2) | 0.999 | 0.629 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | The crystal was grown at 4 degrees C in PACT condition B12 (0.01 M zinc chloride, 0.1 M MES pH 6, 20 % PEG 6000). |
