9TP6

Structure of LolA3 from Porphyromonas gingivalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-09-24
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.873129
Spacegroup name	P 32
Unit cell lengths	124.113, 124.113, 44.389
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.030 - 2.320
R-factor	0.2393
Rwork	0.237
R-free	0.28660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.411
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.370	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.181	9.990
Rmeas	0.190	11.701
Rpim	0.059	4.041
Total number of observations	404859
Number of reflections	39496	1993
<I/σ(I)>	4.3
Completeness [%]	100.0
Redundancy	10.3	8.3
CC(1/2)	0.993	0.523

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.2M Sodium formate, 0.2M Ammonium acetate, 0.2M Sodium citrate tribasic dihydrate, 0.2M Potassium sodium tartrate tetrahydrate, 0.2M Sodium oxamate, Sodium HEPES; MOPS, pH 7.5, 40% v/v PEG 500 MME, 20% w/v PEG 20000