9TP2

HomA ecto-domain mutant H350A E351A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	141.120, 44.600, 68.920
Unit cell angles	90.00, 111.56, 90.00

Refinement procedure

Resolution	42.230 - 1.400
R-factor	0.2088
Rwork	0.209
R-free	0.23000
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.831
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.600	1.480
High resolution limit [Å]	1.400	1.400
Number of reflections	78271	711
<I/σ(I)>	13.5
Completeness [%]	95.9
Redundancy	8.7
CC(1/2)	0.998	0.784

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	18-22% PEG 3350, 0.2M Amonium Iodide