9TOU
Room temperature serial AmpC uncomplexed resting state from PAL-XFEL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | PAL-XFEL BEAMLINE NCI |
| Synchrotron site | PAL-XFEL |
| Beamline | NCI |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2024-06-26 |
| Detector | RAYONIX MX225-HS |
| Wavelength(s) | 1.3042 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.711, 77.647, 98.749 |
| Unit cell angles | 90.00, 116.08, 90.00 |
Refinement procedure
| Resolution | 45.250 - 1.880 |
| R-factor | 0.1863 |
| Rwork | 0.184 |
| R-free | 0.22270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.850 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.700 | 1.912 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Number of reflections | 65589 | 3283 |
| <I/σ(I)> | 2.186 | 0.539 |
| Completeness [%] | 100.0 | 99.94 |
| Redundancy | 25.53 | 17.35 |
| CC(1/2) | 0.926 | 0.069 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 293 | Potassium phosphate (1.9 M, pH 8.8) with crystal seed |
