9TOT

Fixed target room temperature serial crystal structure of hen egg white lysozyme at DLS I24, 600 s mix with N-acetyl glucosamine.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	293
Detector technology	PIXEL
Collection date	2025-06-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9998
Spacegroup name	P 43 21 2
Unit cell lengths	79.164, 79.164, 38.113
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.980 - 1.640
R-factor	0.1581
Rwork	0.157
R-free	0.18700
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.005
RMSD bond angle	0.788
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHENIX
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.980	1.668
High resolution limit [Å]	1.640	1.640
Number of reflections	15382	760
<I/σ(I)>	7.597	0.835
Completeness [%]	100.0	100
Redundancy	434.56	203.2
CC(1/2)	0.056	0.388

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		295	1 M citric acid, 20% (w/v) NaCl, 5% (w/v) PEG 6000, pH 3.0.