9TOR

Drop on fixed target room temperature serial crystal structure of hen egg white lysozyme, drop on fixed target at DLS, 1.9 s mix with N-acetyl glucosamine.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	293
Detector technology	PIXEL
Collection date	2025-06-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9998
Spacegroup name	P 43 21 2
Unit cell lengths	78.983, 78.983, 38.288
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.850 - 1.690
R-factor	0.1805
Rwork	0.179
R-free	0.21380
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.006
RMSD bond angle	0.801
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHENIX
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.850	1.719
High resolution limit [Å]	1.690	1.690
Number of reflections	14077	692
<I/σ(I)>	2.308	1.004
Completeness [%]	100.0
Redundancy	65.86
CC(1/2)	0.988	0.338

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		295	1 M citric acid, 20% (w/v) NaCl, 5% (w/v) PEG 6000, pH 3.0.