9TOQ

Room temperature serial crystal structure of hen egg white lysozyme uncomplexed resting state at DLS I24 on silicon chips

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	293
Detector technology	PIXEL
Collection date	2025-06-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	79.233, 79.233, 38.216
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.030 - 1.650
R-factor	0.176
Rwork	0.174
R-free	0.20480
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.005
RMSD bond angle	0.790
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHENIX
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.030	1.679
High resolution limit [Å]	1.650	1.650
Number of reflections	15167	735
<I/σ(I)>	5.258	0.362
Completeness [%]	100.0	100
Redundancy	270.41	133.79
CC(1/2)	0.997	0.536

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		295	1 M citric acid, 20% (w/v) NaCl, 5% (w/v) PEG 6000, pH 3.0