9TKV
Structure of duck RIG-I (delta CARDS) bound to 31-mer RNA mismatched hairpin with 5'pppG-C blunt end, mimicking the influenza B virus vRNA promoter (panhandle).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 87.641, 100.072, 192.287 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 96.144 - 1.488 |
| Rwork | 0.147 |
| R-free | 0.19140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.387 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.144 | 1.612 |
| High resolution limit [Å] | 1.488 | 1.488 |
| Rmerge | 0.098 | 1.577 |
| Number of reflections | 112184 | 5609 |
| <I/σ(I)> | 13.8 | 1.5 |
| Completeness [%] | 81.3 | 19.2 |
| Redundancy | 8.7 | 9.3 |
| CC(1/2) | 0.999 | 0.541 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | ADA 0.1 M MgCl2 0.1M PEG 6000 12 % ZnCl2 0.1M |
