9THS

Room temperature structure of Ascorbate Peroxidase at 1.54A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	298.7
Detector technology	PIXEL
Collection date	2025-09-09
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.5417
Spacegroup name	P 42 21 2
Unit cell lengths	82.831, 82.831, 76.017
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.019 - 1.540
Rwork	0.167
R-free	0.20130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	1.939
Data reduction software	CrysalisPro
Data scaling software	CrysalisPro
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.105))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.290	1.540
High resolution limit [Å]	1.540	1.540
Number of reflections	42926	2058
<I/σ(I)>	8.8
Completeness [%]	99.9
Redundancy	11.3
CC(1/2)	0.997	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298.7	0.1M Tris HCl, 2.7M Magnesium Sulphate