9THR

Crystal structure of the human serum transferrin with Fe(III) bound at the C-lobe only (treated with DMSO)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-02-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	C 2 2 21
Unit cell lengths	137.098, 156.774, 107.108
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.320 - 3.200
Rwork	0.180
R-free	0.24910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.567
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.320	3.250
High resolution limit [Å]	3.200	3.200
Number of reflections	18284	914
<I/σ(I)>	12.4
Completeness [%]	93.7
Redundancy	10.6
CC(1/2)	0.995	0.921

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	15% (w/v) PEG 3350, 16% (v/v) glycerol, 8 mM disodium malonate, 150 mM Na-PIPES pH 6.5