9TB9
Crystal structure of the CsPYL1 K199S-ABA-HAB1 ternary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-05-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.714, 63.546, 187.628 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.570 - 1.840 |
| R-factor | 0.2516 |
| Rwork | 0.249 |
| R-free | 0.29790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.581 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.910 | 1.900 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.152 | |
| Number of reflections | 35948 | 1745 |
| <I/σ(I)> | 6 | |
| Completeness [%] | 77.7 | |
| Redundancy | 11.9 | |
| CC(1/2) | 0.830 | 0.422 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7 | 291 | 0.1 M Bis-Tris propane, pH 7.0, 0.5 M CaCl2, 25% PEG 3350 |
