9T9C

Shewanella oneidensis EF-Tu-T62A:GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-09
Detector	DECTRIS EIGER2 X CdTe 16M
Wavelength(s)	0.97626
Spacegroup name	P 21 21 21
Unit cell lengths	63.788, 76.923, 97.892
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.950 - 1.360
R-factor	0.1903
Rwork	0.189
R-free	0.21940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.769
Data reduction software	XDS (Jun 39, 2023 (BUILT 20230630))
Data scaling software	STARANISO (2.3.74)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.484	1.488
High resolution limit [Å]	1.359	1.359
Rmerge	0.099	1.638
Number of reflections	62952	3149
<I/σ(I)>	13.9	1.6
Completeness [%]	91.9	56
Redundancy	11.8	12
CC(1/2)	0.999	0.729

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.18 M Sodium Acetate, 21.7 % PEG 3350