9T7U

Glyceraldehyde-3-phosphate dehydrogenase A (GAPDH)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 43 21 2
Unit cell lengths	98.901, 98.901, 252.217
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.830 - 1.700
Rwork	0.152
R-free	0.20940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.777
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.830	1.730
High resolution limit [Å]	1.700	1.700
Number of reflections	137953	93775
<I/σ(I)>	14.3
Completeness [%]	100.0
Redundancy	14.1	13.9
CC(1/2)	0.999	0.612

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	200 mM sodium acetate, 100 mM Bis Tris propane, pH 6.5, and 20% (w/v) PEG 3350