9T7F

Crystal structure of human Signal Regulatory Protein 2 (SIRP) alpha V2 in complex with 5-Hydroxy-L-Tryptophan

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	P 41 21 2
Unit cell lengths	57.457, 57.457, 198.156
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.540 - 1.500
R-factor	0.23279
Rwork	0.232
R-free	0.25306
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.291
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.700	4.490	1.590
High resolution limit [Å]	1.500	3.180	1.500
Rmeas	0.108		1.167
Number of reflections	54272	3840	8602
<I/σ(I)>	19.23
Completeness [%]	100.0
Redundancy	25
CC(1/2)	1.000	0.999	0.666

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.01M Zinc sulfate heptahydrate 0.1M MES monohydrate pH 6.5 25% v/v PEG monomethyl ether 550