9T31
Spindlin family member 4 with crystallization epitope mutations G129S:H131E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92819 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.780, 61.950, 84.900 |
| Unit cell angles | 90.00, 96.86, 90.00 |
Refinement procedure
| Resolution | 53.400 - 2.040 |
| R-factor | 0.2648 |
| Rwork | 0.263 |
| R-free | 0.30050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.980 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.580 | 2.060 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.084 | 0.706 |
| Number of reflections | 36696 | 2726 |
| <I/σ(I)> | 6.6 | |
| Completeness [%] | 98.4 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277.15 | 25% PEG3350 0.1M tris pH 8.5 |
