9T2H
Jumonji domain-containing protein 2B with crystallization epitope mutations L916G:R917A:A918D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92819 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 91.040, 91.040, 102.260 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 62.440 - 1.990 |
| R-factor | 0.2692 |
| Rwork | 0.269 |
| R-free | 0.27950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.880 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.470 | 2.010 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.079 | 2.319 |
| Number of reflections | 18610 | 1348 |
| <I/σ(I)> | 20.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 18.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.15M DL- malic acid 20% PEG3350 |
