9T2G
Human PRKCBP1 zinc finger MYND-type containing 8 with crystallization epitope mutations N221R:M226H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92819 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.802, 71.929, 72.529 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.920 - 1.660 |
| R-factor | 0.2388 |
| Rwork | 0.237 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.960 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.530 | 1.700 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.150 | 3.212 |
| Number of reflections | 42980 | 3108 |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 99.5 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277.15 | 0.2M lithium sulfate 25% PEG3350 0.1M bis-tris pH 6.5 |
